规格
Accession Number:	NM_005226.2
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	293T (HEK)
Cell State:	Dividing Cells
Druggable Target:	GPCRs
Gene Symbol:	EDG3
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	EDG3
Technique:	FRET
Detection Method:	Fluorescent

储存

Contents:
K1713 GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells
• GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293TAssay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Receptor 3 (EDG3) stably integrated into the GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cell line. GeneBLAzer® Gα15-NFAT-bla HEK293T cells (Cat. no. K1539) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.

The GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells are functionally validated for Z' and EC₅₀ concentrations of S1P. In addition, GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

EDG-3/S1P-3(Sphingosine-1-Phosphate-3) is a Gq/Gi/Go/Gα13/Gα12 coupled GPCR. EDG-3 has been shown to be responsible for bradycardia that has been induced in clinical trials as a side effect of the immunosuppressive drug FTY720 that targets EDG-1. EDG-3 has also been shown to induce vasodilation in response to sphingosylphosphorylcholine (SPC), sphingosine-1-phosphate (S1P), and lysosulfatide (LSF). The EDG-3 Receptor has also been shown to play a cooperative or redundant role in angiogenesis with EDG-1.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

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