规格
|
|
Cell Line:
|
293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F |
|
Classification:
|
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
|
Culture Environment:
|
CO2 |
|
Culture Type:
|
Suspension Cell Culture |
|
Endotoxin Level:
|
< 1 EU/ml |
|
Glucose:
|
High Glucose |
|
HEPES Buffer:
|
No HEPES |
|
Included Antibiotics:
|
No antibiotics |
|
Inorganic Salts:
|
Calcium, Magnesium, Sodium Phosphate |
|
Osmolality:
|
275-295 mOsm/kg |
|
Product Line:
|
Expi293™ |
|
Purity or Quality Grade:
|
Cell Culture Grade |
|
Serum Level:
|
Serum-Free |
|
Sodium Bicarbonate Buffer:
|
Sodium Bicarbonate |
|
Sodium Pyruvate Additive:
|
Sodium Pyruvate |
|
Supplements:
|
No Hypoxanthine, No Thymidine, Insulin-Free, No Vitamin A, No 2-Mercaptoethanol, Dextrose, Pyridoxine HCl |
|
Form:
|
Liquid |
|
Glutamine:
|
GlutaMAX™-I |
|
Phenol Red Indicator:
|
No Phenol Red |
|
Cell Type:
|
Kidney (Embryonic) |
|
Concentration:
|
1 X |
|
Format:
|
Bottle(s) |
|
Sterility:
|
Sterile |
储存
|
|
Store in the dark at +2°C to +8°C |
Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.
• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red
Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
Product use
Customers using Expi293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).