规格
Assay:	Tyrosine Kinase Assay
Assay Entry:	Biochemical phospho-specific activity (no antibody)
Excitation/Emission:	405⁄465, 535
No. of Reactions:	800 Reactions
Product Line:	Z´-LYTE®
Readout:	End Point
Research Category:	Signal Transduction
Shipping Condition:	Dry Ice
Substrate Properties:	Peptide-Based Substrate
Target Entry:	PDGFRA T674I, PDGFRB, FGFR1, FGFR2, EGFR, FGFR4, FLT1, MUSK, JAK3, FLT4, FGFR3, INSRR, PDGFRA D842V, EPHA2, PDGFRA, PDGFRA V561D
Target Enzyme:	Tyrosine Kinases
Technique:	FRET
Validated Kinases:	EGFR, EPHA2, FGFR, FLT1, FLT4, INSRR, JAK3, MUSK, PDGFRA, PDGFRB
Label or Dye:	Methylcoumarin
Format:	384-well plate

储存
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

描述

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains an X-(E⁄D)-Y-(I⁄L⁄V) consensus sequence, where X is any residue.

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