PRINCIPLE OF THE ASSAY
This assay employs the quantitative indirect enzyme immunoassay technique. Semaglutide has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-Semaglutide present is bound by the immobilized protein. After washing away any unbound substances, a HRP conjugated probe is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Semaglutide bound in the initial step. The color development is stopped and the intensity of the color is measured.
| Stability and Storage | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃. |
| Detection method | Colorimetric |
| Sample type | Plasma, Serum |
| Assay type | Quantitative |
| Sensitivity | 6.49 ng/mL |
| Range | 15.63 - 10,000 ng/mL |
| Recovery | 80-120% |
| Background | |
| Alternative Names | CAS, 910463-68-2, Semaglutide, Rybelsus, Ozempic, NN9535, OG217SC, NNC 0113-0217 |
| Shipping | 2-8 ℃ |
| Specifications | Semaglutide |
| Note | For Research Use Only. |