Alanine Racemase from Bacillus stearothermophilus,100UNITS,SAE0023-100UN,Sigma

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订货号 0BL2677
品牌型号 Sigma SAE0023-100UN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

Application

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.


Biochem/physiol Actions

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†


Physical form

Aqueous solution containing 10 mM potassium phosphate


Other Notes

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.


技术参数 Specifications
biological sourceBacillus sp. (B. stearothermophilus)
Quality Level200
formbuffered aqueous solution
specific activity≥10 IU/mg solid
mol wtMr 78 kDa (2 subunits 39 kDa each)
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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