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General description
High affinity immunoglobulin gamma Fc receptor I (UniProt: P12314; also known as IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64) is encoded by the FCGR1A (also known as FCG1, FCGR1, IGFR1) gene (Gene ID: 2209) in human. CD64 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-15), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 16-292), a transmembrane domain (aa 293-313), and a cytoplasmic domain (aa 314-374). Within its extracellular domain are three Ig-like C2 type domains. Its expression has been reported in monocytes/macrophages. Expression of CD64 distinguishes conventional dendritic cells from monocyte-derived dendritic cells. It serves as a high-affinity receptor for the Fc domains of IgG (Fc R) and plays a key role in linking the humoral and the cellular arms for the immune system. It binds human IgG1, IgG3, and IgG4 with a high affinity, but does not show any affinity for IgG2. It functions in both innate and adaptive immune responses. Along with the low affinity receptor (Fc RII), it mediates a variety of biological responses, including endocytosis, phagocytosis, antibody-directed cellular cytotoxicity, and cytokine release. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Harrison, PT., and Allen., (1998). Protein Engineering. 11 (3); 225-232; Dougherty, GJ., et al. (1987). Eur. J. Immunol. 17(10); 1453-1459).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the extracellular domain of human CD64.
Application
Quality Control Testing
Evaluated by Western Blotting in Human tonsil tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD64 in Human tonsil tissue lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound CD64 peptide with a KD of 3.6 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD64 in one million U937 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD64 in U937 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1B5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects High affinity immunoglobulin gamma Fc receptor I (CD64). It targets an epitope within 21 amino acids from the extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B5, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 43 kDa,observed mol wt ~43 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_000557 |
| UniProt accession no. | P12314 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... FCGR1A(2209) |
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