- 商品介绍
- 规格参数
- 包装参数
Application
Tumor Necrosis Factor Soluble Receptor II (TNF sRII)/Fc Chimera human can be used as a test compound for analyzing the interaction between tumor necrosis factor (TNF) and its two soluble receptors by using surface plasmon resonance (SPR) technique.
Biochem/physiol Actions
Tumor Necrosis Factor Soluble Receptor II (TNF sRII)/Fc Chimera human belongs to TNF family of receptors and mediates the metabolic effects of TNF-α. Tumor Necrosis Factor Soluble Receptor II blocks the TNF-α-induced apoptosis and hence regulates the TNF-α function. It also has a pivotal role in neutralizing the biological activities of both TNF-α and TNF-β.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline
Analysis Note
The biological activity is measured by its ability to neutralize the TNF-α mediated cytotoxicity in mouse L-929 cells in the presence of the metabolic inhibitor actinomycin D.
| biological source | human |
| Quality Level | 200 |
| recombinant | expressed in NSO cells |
| assay | >95% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.004-0.016 mg per mL ED50 |
| mol wt | 52.6 kDa |
| packaging | pkg of 50 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | P20333 |
| storage temp. | −20°C |
| Gene Information | human ... TNFRSF1B(7133) |
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