- 商品介绍
- 规格参数
- 包装参数
Application
Desthiobiotin-NHS may be used in protein modification, bioconjugation, and pull-down assays.
Biochem/physiol Actions
Desthiobiotin-NHS, an amine-reactive NHS tag, facilitates efficient and reversible protein enrichment. The desthiobiotin handle, a structural analog of biotin lacking a sulfur atom, exhibits a reduced binding affinity for avidin/streptavidin (µM KD) compared to biotin (pM KD), enabling gentle elution through competitive displacement with biotin or by weakening the interaction with mild alkali, thereby preserving protein integrity. This NHS tag readily conjugates to protein amines, enabling flexible, multi-cycle binding, washing, and elution. Note: the lower affinity of the desthiobiotin handle requires careful optimization to minimize non-specific interactions and potential loss during washes compared to biotin-streptavidin systems.
| Quality Level | 100 |
| assay | ≥95% (HPLC) |
| form | powder |
| color | white to beige |
| solubility | DMSO: 2 mg/mL, clear |
| storage temp. | -10 to -25°C |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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NALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/8 x 1/4 x 1/16，英寸，每箱数量50，50/箱，8000-0020，Thermofisher，赛默飞世尔询价
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