邻苯二胺 二盐酸盐，50TABLETS，P7288-50TAB，Sigma

Application

o-Phenylenediamine dihydrochloride has been used:

• as a substrate for the eosinophil peroxidase (EPO) assay in bronchoalveolar lavage fluid (BALF)
• in cell adhesion molecule-enzyme immunoassay (CAM-EIA) to analyze the amounts of E-selectin induced by test compounds (LPS or solutions and materials) on endothelial cells
• in ultra-sensitive enzyme-linked immunosorbent assay (ELISA) to measure prolactin and luteinizing hormone (LH)

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.