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- 规格参数
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General description
Protein unc-13 homolog B (UniProt: O14795; also known as Munc13-2, munc13) is encoded by the UNC13B (also known as UNC13) gene (Gene ID: 10497) in human. Protein plays a critical role in synaptic transmission and neurotransmitter release. It is involved in priming synaptic vesicles for exocytosis, acting as a key regulator in the docking and fusion of vesicles with the presynaptic membrane. Munc13-2/UNC13B interacts with various SNARE proteins, which are essential for the fusion of vesicles and the release of neurotransmitters into the synaptic cleft. It is highly expressed in the brain, especially in the cerebral cortex, where it influences neuronal excitability. It is also expressed in kidney cortical epithelial cells. Munc13-2 is localized to the presynaptic terminals of neurons and is also translocated to the plasma membrane. Munc13-2 is characterized by several distinct domains, including a C2 domain, a Munc13 homology domain, and a C1 domain. The C2 domain is involved in calcium binding, which is crucial for its role in synaptic transmission. The Munc13 homology domain is important for interactions with other proteins involved in exocytosis, while the C1 domain is associated with the binding of diacylglycerol, a lipid that plays a role in signaling pathways. Two isoforms of Munc13-2 have been described that are produced by alternative splicing. Alterations in Munc13-2 function or expression have been implicated in several neurological disorders. mutations in the Munc13-2 gene have been associated with epilepsy and other synaptic dysfunctions. Additionally, dysregulation of Munc13-2 has been linked to neurodegenerative diseases, such as Alzheimer s disease, where impaired neurotransmitter release may contribute to cognitive decline. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.; Ansari, U., et.al. (2023). AIMS Neurosci. 6;10(4);388-400).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human UNC13B.
Application
Quality Control Testing
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected UNC13B in MCF7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected UNC13B in lysates from HEK-293T cells and human brain tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected UNC13B in human kidney tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound UNC13B recombinant protein with at least one thousand-fold (1,000X) higher affinity than with non-specific GST control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1B23 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects UNC13B. It targets an epitope within 23 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B23, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 180.68 kDa,observed mol wt ~200.00 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_006368 |
| UniProt accession no. | O14795 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... UNC13B(10497) |
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