- 商品介绍
- 规格参数
- 包装参数
General description
Isoelectric point : 4.6
Michaelis constants : 1.1 X 10-3M (NH3), 3.4 X 10-4M (α-Ketoglutarate)
1.2 X 10-3M (L-Glutamate), 1.4 X 10-5M (NADPH), 1.5 X 10-5M (NADP+)
Structure : 6 subunits (M.W.50,000) per mol of enzyme
Inhibitors : Hg++, Cd++, p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine,
2,2′-dithiopyridine
Optimum pH : 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG)
Optimum temperature : 45oC(α-KG−L-Glu) 45-55oC (L-Glu→α-KG)
pH stability : pH 6.0 - 8.5 (25oC, 20hr)
Thermal stability : below 50oC (pH 7.4, 10min)
Application
This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis. In vitro, various activity assays of this enzyme examine the conversion of α-ketoglutarate to
Biochem/physiol Actions
L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.
Physical form
Solution in 50 mM Tris HCl, pH 7.8, 5 mM Na2EDTA containing 0.05% sodium azide
Other Notes
Note: Do not confuse with non-specific
One unit will reduce 1.0 μmole of α-ketoglutarate to
| biological source | bacterial (Proteus spp.) |
| Quality Level | 200 |
| form | buffered aqueous solution |
| specific activity | ≥4,000 units/mL |
| mol wt | ~300 kDa |
| storage temp. | 2-8°C |
| 长度(mm) | |
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¥ 6330.00
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmPES滤膜，孔径0.45um,适合瓶颈尺寸33mm，12/箱，295-3345，Nalgene，Thermofisher，赛默飞世尔¥ 1938.54
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¥ 1156.91
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¥ 4011.97
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¥ 5014.59
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¥ 1586.69
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¥ 2099.27
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¥ 3476.87
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¥ 9909.51
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