Monoclonal Anti-CD22 PePerCP-Cyanine5.5 produced in mouse，100YL4，SAB4702132-100TST，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Immunogen

Whole hairy cell leukemia cells and membrane preparation

Biochem/physiol Actions

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Physical form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

技术参数 Specifications

conjugate	PerCP-Cy5.5 conjugate
Quality Segment	100
antibody product type	primary antibodies
clone	S-HCL-1, monoclonal
form	buffered aqueous solution
species reactivity	human
technique(s)	flow cytometry: suitable
isotype	IgG2bκ
UniProt accession no.	P20273
storage temp.	2-8°C
target post-translational modification	unmodified
Gene Information	human ... CD22(933)

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