BAY-850 trihydrochloride，25MG，SML2748-25MG，Sigma

Biochem/physiol Actions

BAY-850 is a chemical probe that targets ATPase family AAA domain-containing protein 2 (ATAD2) bromodomain (BD) with submicromolar affinity (K_d of 84.9 nM by MST and 120 nM by BROMOscan) and high selectivity over all other bromodomains tested (no BROMOscan hits at 10 μM). BAY-850 blocks ATAD2 BD from binding acetylated H4 peptide in cell-free assays (IC₅₀ of 20 nM) and displaces full-length ATAD2 from chromatin in live cells (effctive conc. 1 μM by HTRF). To avoid potential unspecific off-target effects, concentrations above 5 μM are not recommended. BAY-460 is a companion control compound with strongly reduced ATAD2 inhibitory potency (IC₅₀ = 16 μM by cell-based HTRF assay; no activity at 10 μM by BROMOscan).
Chemical probe that targets ATAD2 bromodomain (BD) with submicromolar affinity and displaces full-length ATAD2 from chromatin in live cells.