Bisindolylmaleimide X hydrochloride，1MG，B3931-1MG，Sigma

Application

Bisindolylmaleimide X hydrochloride has been used as a protein kinase C (PKC) inhibitor:

• in chemotaxis assays
• to inhibit protein kinase C and to study its effects on the expression of EGR1, NAB2, ZEBRA, and Rta
• in the culture to study its effects on the expression of the kinase-insert domain-containing receptor (KDR)-B1, protein kinase Cθ (PKCθ)-M1a or Abl-HTa and on cell yield in baculovirus (BV)-infected insect cells

Biochem/physiol Actions

Bisindolylmaleimide X hydrochloride/Ro 31-8425, a strong and selective protein kinase C (PKC) inhibitor, can reduce the superoxide burst caused by various agonists in neutrophils. It can suppress the responses induced by cell surface receptors and phorbol esters in T cells. In humans, Ro 31-8425 can prevent neutrophil PKC in vitro with an IC50 of 5nM.