5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt,1MG,B0631-1MG,Sigma

销售价: ¥ 1750.19 / 件
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订货号 0BS3917
品牌型号 Sigma B0631-1MG
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.


Application

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:

  • in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells
  • to label cleaved DNA ends in apoptosis assay in avian tissue sections
  • for incorporation into mice prostates for immunohistochemistry studies


5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.


技术参数 Specifications
biological sourcesynthetic (organic)
Quality Level200
assay≥90%
formpowder
solubilitywater: 50 mg/mL, clear, colorless to faintly yellow
storage temp.−20°C
SMILES string[Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=C(Br)C(=O)NC2=O
InChI1S/C9H14BrN2O14P3.Na.H/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI keyUWZGQOWZUSBZMC-UHFFFAOYSA-N
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