DSSO-Carbamate，50MG，SML4369-50MG，Sigma

Biochem/physiol Actions

DSSO-carbamate is an advanced crosslinker optimized for cross-linking mass spectrometry (XL-MS) applications, offering superior chemical stability and reactivity compared to DSSO. By replacing ester groups with carbamates, it resists retro-Michael degradation, ensuring higher recovery rates for crosslinks and monolinks under physiological conditions. This enhanced performance facilitates precise structural mapping of flexible and multiconformational proteins, significantly improving integration with computational tools like AlphaFold2. DSSO-carbamate’s improved sensitivity, stability, and structural accuracy make it a valuable addition for proteomics, particularly in resolving dynamic protein conformations and interactions.