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General description
The p53 protein was first identified in a complex with the simian virus 40 (SV40) large T-antigen.4-5 p53 is classified as a key tumor suppressor and is the most commonly mutated gene in human cancers; it is mutated in over 50% of all human cancers.6 Due to its central role in the DNA damage response (DDR), p53 is often referred to as the “guardian of the genome” and is suggested to be the most studied human gene of all time.7-8 p53 mutations are frequent in breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma and certain types of leukemia1-8.
Application
The antibody may be used in various immunochemical techniques including Immunoblotting (~53 kDa), Immunofluorescence, Immunoprecipitation1, Immunohistochemistry2 and ChIP3.
Biochem/physiol Actions
Monoclonal Anti-p53 antibody specifically recognizes a denaturation-resistant epitope on the primate p53 nuclear protein and does not react with other cellular proteins."
The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novelmarker for malignancy.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.Antibody Concentration: ∼ 1.0 mg/mL
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| conjugate | unconjugated |
| Quality Segment | 200 |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | BP53-12, monoclonal |
| species reactivity | human |
| concentration | ~1 mg/mL |
| technique(s) | immunoblotting: 0.06-0.125 μg/mL using human epidermoid carcinoma A431 cell line whole extract,immunofluorescence: 0.25-0.5 μg/mL using human A431cells |
| isotype | IgG2a |
| UniProt accession no. | P04637 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TP53(7157) |
| 长度(mm) | |
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NALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/8 x 1/4 x 1/16，英寸，每箱数量50，50/箱，8000-0020，Thermofisher，赛默飞世尔
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