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- 规格参数
- 包装参数
General description
Luciferin 4-monooxygenase (UniProt: P08659; also known as EC: 1.13.12.7, Luciferase) is a peroxisomal enzyme that belongs to the ATP-dependent AMP-binding enzyme family. It catalyzes the production of oxyluciferin from luciferin in an ATP and magnesium dependent reaction and releases a photon of light when oxyluciferin returns to the ground state. This reaction proceeds in two steps where the first step involves adenylation of luciferin and in the second step luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide, the decay of oxyluciferin producing a photon of light of 562 nm. Luciferase from the firefly is commonly used in reporter assay where the total light emission is proportional to the luciferase activity of the sample.
~58 kDa observed; 60.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: unknown
GST-tagged full length recombinant firefly luciferase.
Application
Anti-Luciferase, Cat. No. AB3256-I, is a rabbit polyclonal antibody that detects firefly luciferase and has been tested for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Luciferase in HeLa cells transfected with Luciferase expression plasmid.
Research Category
Secondary & Control Antibodies
Biochem/physiol Actions
This rabbit polyclonal antibody detects firefly luciferase.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in lysate from HeLa cells transfected with Luciferase expression plasmid.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Luciferase in HeLa cells transfected with luciferase expression plasmid.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity (predicted by homology) | all |
| packaging | antibody small pack of 25 μL |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| web metadata keyword.default | Anti-Luciferase antibody Sigma-Aldrich,Anti-Luciferase for ATP-dependent reactions,Anti-Luciferase for enzyme activity detection,Anti-Luciferase for immunocytochemistry applications,Anti-Luciferase for luciferin studies,Anti-Luciferase for luminescence assays,Anti-Luciferase monoclonal antibody,Anti-Luciferase western blot compatible,Anti-Luciferase,EC 1.13.12.7 Anti-Luciferase antibody,Luciferin 4-monooxygenase antibody P08659,high purity Anti-Luciferase for research,purified Anti-Luciferase protein,recombinant Anti-Luciferase antibody |
| UniProt accession no. | P08659 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | Photinus pyralis ... Loc116160065(116160065) |
| 长度(mm) | |
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