- 商品介绍
- 规格参数
- 包装参数
General description
~58 kDa observed; 57.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Regulator of nonsense transcripts 3B (UniProt: Q9BZI7; also known as Nonsense mRNA reducing factor 3B, Up-frameshift suppressor 3 homolog B, UPF3B, hUpf3B, Up-frameshift suppressor 3 homolog on chromosome X, hUpf3p-X) is encoded by the UPF3B (also known as RENT3B, UPF3X) gene (Gene ID: 65109) in human. UPF3B is widely expressed, including in testis, uterus, heart, muscle, brain, spinal cord, and placenta. It shuttles between the nucleus and the cytoplasm. It is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serves as a link between the EJC core and NMD machinery. UPF3B recruits UPF2 at cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex is believed to activate NMD. In cooperation with UPF2 it stimulates both ATPase and RNA helicase activities of UPF1. Mutations in UPF3B gene have been linked to X-linked mental retardation that is characterized by significantly low general intellectual functioning associated with impairments in adaptive behavior, autistic features, poor musculature and other abnormalities. Two isoforms of UPF3B have been described that are produced by alternative splicing.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human Up-frameshift suppressor 3 homolog B.
Epitope: unknown
Application
Anti-UPF3B, Cat. No. ABE1900, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 3B (UPF3B) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected UPF3B in lysate from HeLa cells overexpressing UPF3B. (Courtesy of Matthew Kim & Dr. Wilkinson Miles at University of California, San Diego).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected UPF3B in human testis and mouse testis tissue sections.
Western Blotting Analysis: 2 µg/mL from a representative lot detected UPF3B in lysate from HEK cells transfected with UPF3B vector, but not in HEK cells transfected with control vector.
Biochem/physiol Actions
This rabbit polyclonal antibody detects human Up-frameshift suppressor 3 homolog B (UPF3B). It targets an epitope with in 18 amino acids from the C-terminal half.
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in iPSCs and UPF3B-KO iPSCs.
Western Blotting Analysis: 2 µg/mL of this antibody detected UPF3B in lysate from iPSCs, but not in lysate from UPF3B knockout iPSCs.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_542199 |
| UniProt accession no. | Q9BZI7 |
| target post-translational modification | unmodified |
| Gene Information | human ... UPF3B(65109) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 12156.68
-
询价
-
询价
-
¥ 1596.77
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 3372.68
-
¥ 1653.62
-
询价
-
询价
-
询价
-
询价
-
¥ 3319.60
-
¥ 2307.00
-
¥ 1564.00
微信小程序
7X24小时在线咨询