ABTS™ Chromophore,100ML,194434-100MLCN,Sigma

销售价: ¥ 2259.56 / 件
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订货号 0BV4398
品牌型号 Sigma 194434-100MLCN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.
Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.


Physical form

Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.


Preparation Note

ABTS solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.


Other Notes

Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.


Legal Information

ABTS is a trademark of Boehringer Mannheim Corp.
ABTS is a trademark of Roche
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Irritant (B)


技术参数 Specifications
biological source (Synthetic chemical)
Quality Level100
formsolution
contains≤0.1% sodium azide as preservative
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze
colorclearvery pale yellow
shipped inambient
storage temp.2-8°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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