GSK-3抑制剂IX，10MG，361550-10MGCN，Sigma

General description

The small molecule, 6-bromoindirubin-3′-oxime (BIO) serves as a specific inhibitor of glycogen synthase kinase-3 (GSK-3). It can sustain self-renewal and pluripotency in both human and mouse embryonic stem cells (ESCs). Treating ESCs with BIO increases β-catenin activity, suggesting that the activation of canonical Wnt signaling plays a key role in preserving the stem cell characteristics. BIO can also induce adult mammalian cardiomyocytes to dedifferentiate and enter mitosis. In addition to its effects on stem cells, the GSK-3 inhibitor IX (BIO) has the ability to elevate the expression of caspase-3 and caspase-8 proteins, leading to the induction of apoptosis in glioblastoma (GBM) cells. This GSK-3 inhibitor also triggers G2/M cell cycle arrest in GBM cells.

Application

BIO has been used as a GSK-3 inhibitor:

• in aortic valve interstitial cells (VICs) culture to induce osteogenesis in VICs
• to treat VICs to study its effects on RUNX2, GSK-3β, and p-SMAD protein expression
• and a Wnt pathway activator to study the effect of Wnt and bone morphogenetic protein (BMP) pathways on Saos-2 using differentiation markers Dlx5, Runx2 and Msx2

Biochem/physiol Actions

产物与ATP竞争。
可逆性：是
细胞可渗透性：是

Packaging

用惰性气体包装

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性：致癌/致畸（D）