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General description
Uromodulin (UniProt: P07911; also known as Tamm-Horsfall urinary glycoprotein, THP) is encoded by the UMOD gene (Gene ID: 7369) in human. Uromodulin is a homodimeric glycoprotein that is produced in the kidney and can polymerize into long filaments. It is expressed in the tubular cells of the kidney and is most abundant protein in normal urine. It plays a role in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle′s loop, where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. It also facilitates neutrophil migration across renal epithelia. Uromodulin contains a ZP domain (aa 334-589) that mediates polymerization, leading to the formation of long filaments. The core of the filament consists of stacked ZP domains, which assemble into a helical structure. Each ZP domain consists of an N-terminal (ZP-N; aa 334-429) and C-terminal (ZP-C; aa 465-589) region connected by a flexible linker. The linker allows the ZP domain to wrap around the ZP-C subdomain of the preceding subunit. It is proteolytically cleaved at a conserved C-terminal proteolytic cleavage site to generate the secreted form found in the urine. In the urine, it may contribute to colloid osmotic pressure, retard passage of positively charged electrolytes, and prevent urinary tract infection. It also inhibits the formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Five isoforms of uromodulin have been described that are produced by alternative splicing. (Ref.: Micanovic, R., et al. (2022). Am. J. Physiol. Renal Physiol. 322(4); F403-418; Schmid, M., et al. (2010). Cell Physiol. Biochem. 26(3); 311-318; Santambrogio, S., et al. (2008). Biochem. Biophys. Res. Commun. 370(3); 410-413).
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of Human Uromodulin/THP.
Application
Quality Control Testing
Evaluated by Western Blotting in Human kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Uromodulin/THP in Human kidney tissue lysate.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Uromodulin/THP in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
This rabbit polyclonal antibody detects Uromodulin/THP. It targets an epitope within 22 amino acids from the C-terminal region.
Physical form
Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
Preparation Note
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| mol wt | calculated mol wt 69.76 kDa,observed mol wt ~105 kDa |
| purified by | affinity chromatography |
| species reactivity | human |
| packaging | antibody small pack of 100 μg |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgGκ |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_003352 |
| UniProt accession no. | P07911 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... UMOD(7369) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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