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General description
CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.
The unconjugated parent antibody (Catalog No. MABD122) has an observed MW at 63 kDa
Immunogen
Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)
Application
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
This Anti-CD73 Antibody, clone AA60-E3-3, Cy3 Conjugate is validated for use in Immunocytochemistry for the detection of CD73.
Physical form
Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Preparation Note
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry on human adipose and bone marrow mesenchymal stem cells.
Immunocytochemical analysis: A 1:100 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | CY3 conjugate |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | AA60-E3-3, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (immunogen homology) |
| technique(s) | immunocytochemistry: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001191742 |
| UniProt accession no. | Q61503 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NT5E(4907) |
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