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General description
CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
~220 kDa observed
Immunogen
Recombinant protein corresponding to Human Mi-2β.
Application
Research Sub Category
Chromatin Biology
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was used for ChIP. See Zhang et al. (2012).
ChIP-seq: representative lot of this antibody was used for ChIP-seq. See Zhang et al. (2012).
Research Category
Epigenetics & Nuclear Function
This Anti-Mi-2β Antibody, clone 16G4 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in a Jurkat cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 16G4, monoclonal |
| species reactivity | human |
| technique(s) | ChIP: suitable (ChIP-seq),western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001264 |
| UniProt accession no. | Q14839 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CHD4(1108) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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