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General description
H1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.
~34 kDa observed
Immunogen
Epitope: Acetylated Lys34 and phosphorylated Ser36
KLH-conjugated linear peptide corresponding to human Histone H1 acetylated at Lys34 and phosphorylated at Ser36.
Application
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with acetyl (Lys34) phospho (Ser36) Histone H1.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody (Rabbit Polyclonal Antibody) validated in WB & DB to detect acetyl (Lys34) phospho (Ser36) Histone H1 also known as Histone H1b.
Biochem/physiol Actions
Other homologies: Mouse and Rat (90% sequence homology).
This antibody recognizes Histone H1 acetylated at Lys34 and phosphorylated at Ser36.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Calf thymus histone extract
Evaluated by Western Blot in calf thymus histone extract.
Western Blot Analysis: A 1:37 dilution of this antibody detected Histone H1 on 10 µg of calf thymus histone extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, bovine |
| species reactivity (predicted by homology) | canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology) |
| technique(s) | dot blot: suitable,western blot: suitable |
| NCBI accession no. | NP_005312 |
| UniProt accession no. | P10412 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer36), acetylation (Lys34) |
| Gene Information | bovine ... H1-4(617854) dog ... H1-4(488269) human ... H1-4(3008) rhesus monkey ... H1-4(696872) |
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