Ready-to-Assay PTH1 Parathyroid Hormone Receptor Frozen Cells，1KIT，HTS030RTA，Sigma

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Parathyroid hormone (PTH) plays a critical role in mineral ion homeostasis, particularly in bone and kidney (Mannstadt et al., 1999; Gensure et al., 2005). A related peptide, parathyroid hormone-related peptide (PTHrP), plays an important role in skeletal development. Both peptides exert their biological actions by binding to a class B GPCR, PTH1, that signals primarily by activation of adenylate cyclase (Schipani et al., 1993). Mutations in PTH1 have been identified as the cause of Jansen′s metaphyseal chondrodysplasia, Blomstrand′s chondrodysplasia, and enchondromatosis (Schipani and Provot, 2003). Intermittent treatment with PTH has important clinical utility in building bone mass in patients with osteoporosis (Rosen, 2003). Millipore′s cloned human PTH1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PTH1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PTH1 and its ligands.

Application

Human PTH1 GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

• GPCR Cell Lines
• Host cells: Chem-1

• GPCR Class: B
• Protein Target: PTH1
• Target Sub-Family: PTH receptor

Other Notes

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.

Disclaimer

This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.