SureClick™ Antibody Labeling Kit，SC002，赛默飞世尔

What is SureClick antibody labeling?

SureClick is a site-specific antibody conjugation technology that covalently attaches payloads—including fluorophores, functional tags, and oligonucleotides—to the Fc region of IgG antibodies using click chemistry. Unlike conventional random labeling approaches, SureClick:

• Targets the Fc domain
• Preserves Fab antigen-binding regions
• Maintains Fc glycosylation integrity
• Enables reproducible degree of labeling (DOL)
• Supports consistent antibody–oligonucleotide conjugation

Key features and benefits:

Precise, reproducible labeling

• Site-specific Fc conjugation
• Consistent attachment of ~2 payloads per antibody
• Reduced heterogeneity compared to conventional methods

Rapid two-hour workflow

• ~10 minutes hands-on time
• 90-minute protocol
• Simple protocol using a single Fc-targeting component

Covalent and stable conjugation

• Trusted click chemistry
• Robust linkage for downstream assays, spatial biology, and antibody–oligo workflows

Preserves antibody function

• Confidence that antigen binding is unchanged
• Maintains Fc receptor interactions

Automation-ready

• Spin-column purification
• Minimal components
• Suitable for high-throughput workflows

How SureClick antibody labeling works

1. Add SureClick Fc-targeting reagent to your IgG antibody
2. Purify using included Zeba™ spin columns
3. Add click chemistry payload of choice (e.g., fluorophores, oligonucleotides, functional tags)

The result is a site-specific, covalently labeled antibody with consistent performance, including highly controlled antibody–oligonucleotide conjugates.

Applications

• Spatial biology
• Antibody–oligonucleotide conjugation
• Multiplex biomarker detection
• Antibody-drug conjugate (ADC) development
• Biotherapeutic characterization
• Flow cytometry
• Internalization studies
• Biomarker validation