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General description
Ankyrin repeat domain-containing protein 11 (UniProt: Q6UB99; also known as Ankyrin repeat-containing cofactor 1, ANKRD11) is encoded by the ANKRD11 (also known as ANCO1) gene (Gene ID: 29123) in human. ANKRD11 is a large nuclear protein that is shown to localize to chromatin during prometaphase. Its expression levels are regulated during the cell cycle, reaching maximal levels at M phase and then rapidly declining after late M phase. It contains four Ankyrin repeats in its N-terminal region. ANKRD11 plays an important role during neural development by modulating histone acetylation and gene expression and thus proliferation of embryonic neural precursors and the genesis and positioning of newborn neurons. It may recruit histone deacetylases to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation. Knockdown of ANKRD11 gene in developing murine or human cortical neural precursors results in decreased proliferation, reduced neurogenesis, and aberrant neuronal positioning. Mutations in ANKRD11 have been linked with KBG syndrome that is characterized by macrodontia, distinctive craniofacial features, short stature, skeletal anomalies, global developmental delay, seizures, and intellectual disability. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gallagher D., et al. (2015). Dev. Cell. 32(1); 31-42; Zhang, A., et al. (2004). J. Biol. Chem. 279(32); 33799-33805).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
His-tagged recombinant fragment corresponding to 181 amino acids from the N-terminal half of human Ankyrin repeat domain-containing protein 11 (ANKRD11).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ANKRD11 in HeLa cell nuclear extract.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ANKRD11 in A431 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ANKRD11 in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ANKRD11 recombinant fragment with a KD of 2.9 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ANKRD11 in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1H14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Ankyrin repeat domain-containing protein 11 (ANKRD11). It targets an epitope within the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1H14, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 297.91 kDa,observed mol wt ~200 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_001243111 |
| UniProt accession no. | Q6UB99 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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