Monoclonal Anti-β-Tubulin I+II antibody produced in mouse，25UL，T8535-25UL，Sigma

General description

β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with a core of two β-sheets enclosed by α-helices. In addition, it also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. β-tubulin I and II is encoded by the gene mapped to human chromosome 20 and 6p25.2 respectively.
Monoclonal Anti-β-Tubulin Isotype I + II (mouse IgG2b isotype) is derived from the JDR.3B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

Immunogen

synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype II coupled to BSA

Application

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in following studies:

• immunoblot
• immunofluorescence technique
• immunohistochemistry.

• enzyme linked immunosorbent assay (ELISA)
• immunohistochemistry
• western blotting
• immunoblotting
• dot blot
• immunocytochemistry

Biochem/physiol Actions

β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Tubulin β 2B class IIb (TUBB2B)/β-tubulin II plays a vital role in migration/organization and axon guidance processes. Mutation in β-tubulin gene is associated with the development of asymmetric polymicrogyria (PMG).
The antibody specifically recognizes an epitope located on isotypes I and II of β-tubulin. No cross-reactivity with other tubulin isotypes is observed.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.