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General description
Probable ATP-dependent RNA helicase DDX4 (UniProt: Q9NQI0; also known as EC:3.6.4.13, DEAD box protein 4, Vasa homolog) is encoded by the DDX4 (also known as VASA) gene (Gene ID: 54514) in human. Probable ATP-dependent RNA helicase DDX4, belongs to the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX4 is primarily involved in the regulation of RNA metabolism, germline formation, fertility, and piRNA-dependent retrotransposon control. It plays a critical role in processes such as RNA splicing, ribosome biogenesis, and the regulation of translation. DDX4 is localized mainly in the cytoplasm of germ cells, where it associates with germ granules such as nuage in mammals and pole plasm in Drosophila. These granules are involved in the storage and regulation of mRNAs required for germ cell development and function. Aberrant expression or function of DDX4 can lead to infertility due to defects in germ cell development. Dysregulation of DDX4 gene is associated with reproductive disorders and certain types of germ cell tumors, such as seminomas and dysgerminomas. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gustafson, E. A., et al., 2010, Biochem. Biophy. Res. Commun. 395; 1-6, Xu, C., et al., 2022, Cell. Mol. Life Sci.79; 4)
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human DDX4.
Application
Quality Control Testing
Evaluated by Western Blotting in NTERA-2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DDX4 in NTERA-2 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DDX4 in NTERA-2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DDX4 in human testis tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1J11 is a ZooMAb® rabbit recombinant monoclonal antibody that detects DDX4. It targets an epitope within 21 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1J11, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 79.31 kDa,observed mol wt ~82 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_077726 |
| UniProt accession no. | Q9NQI0 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... DDX4(54514) |
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