BugBuster® GST-Bind Purification Kit，1KIT，70794-3，密理博

销售价： ¥ 6984.63 / 件

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订货号： 0BL4172

品牌型号：密理博 70794-3

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最小订货量： 1件

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商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

Affinity purification of GST fusion proteins
The BugBuster^® GST•Bind Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.

Other Notes

•2 x 100 mlBugBuster Protein Extraction Reagent

•10,000 UBenzonase Nuclease, purity >90%

•10 mlGST•Bind Resin

•pkg/4Chromatography Columns

•2 x 100 ml10X GST Bind/Wash Buffer

•40 ml10X Glutathione Reconstitution Buffer

•1 gGlutathione, Reduced