- 商品介绍
- 规格参数
- 包装参数
General description
The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin.
Immunogen
synthetic peptide corresponding to amino acid residues 884-901 of human INCENP with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in chicken and Xenopus laevis and by two amino acids in mouse.
Application
Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence.
Biochem/physiol Actions
Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis.
The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~106 kDa |
| species reactivity | human, mouse, rat |
| technique(s) | indirect immunofluorescence: 1:100 using HeLa cells,microarray: suitable,western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells,western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells |
| UniProt accession no. | Q9NQS7 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... INCENP(3619) mouse ... Incenp(16319) rat ... Incenp(293733) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
¥ 2622.09
-
询价
-
询价
-
询价
-
询价
-
¥ 8520.40
-
¥ 9737.84
-
¥ 3152.47
-
询价
-
询价
-
询价
-
¥ 4897.21
-
¥ 8982.64
-
¥ 1749.15
-
¥ 745.60
-
¥ 4020.77
-
¥ 2099.27
-
¥ 1351.48
微信小程序
7X24小时在线咨询