- 商品介绍
- 规格参数
- 包装参数
General description
This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.
Application
Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.
Biochem/physiol Actions
Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.
| biological source | human |
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | ≥50% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 137 kDa (mTOR), 150 kDa (Raptor), 37 kDa (MLST8) |
| packaging | pkg of 10 μg |
| manufacturer/tradename | Sigma-Aldrich |
| technique(s) | cell based assay: suitable |
| solubility | water: soluble |
| NCBI accession no. | NM_004958 (mTOR),NM_020761 (Raptor),NM_022372 (MLST8) |
| application(s) | life science and biopharma |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... MLST8(64223), MTOR(2475), RPTOR(57521) |
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¥ 2622.09
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¥ 1779.22
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¥ 3275.78
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¥ 2573.97
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¥ 2268.97
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¥ 1568.60
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¥ 4011.97
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¥ 3404.94
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¥ 2452.91
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