- 商品介绍
- 规格参数
- 包装参数
General description
EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein. It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.
Application
Suitable substrate for MNK1 kinase.
Biochem/physiol Actions
EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.
Physical form
Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥70% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 51 kDa |
| packaging | pkg of 100 μg |
| concentration | >0.02 mg/mL |
| technique(s) | cell based assay: suitable |
| suitability | suitable for molecular biology |
| NCBI accession no. | NM_001968 |
| UniProt accession no. | P06730 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... EIF4E(1977) |
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¥ 12156.68
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¥ 2068.25
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¥ 4173.75
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmSFCA滤膜，孔径0.2um,适合瓶颈尺寸45mm，12/箱，291-4520，Nalgene，Thermofisher，赛默飞世尔¥ 2856.42
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¥ 1476.00
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¥ 1156.91
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¥ 1586.69
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¥ 39793.68
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