Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse,0.5ML,A7554-.5ML,Sigma

销售价: ¥ 4127.83 / 件
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订货号 0BL7293
品牌型号 Sigma A7554-.5ML
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.


Immunogen

Purified bovine IgG


Application

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:

  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunohistochemistry


Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.
bovine IgG1 and IgG2


Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcemouse
Quality Level200
conjugatealkaline phosphatase conjugate
antibody formpurified immunoglobulin
antibody product typesecondary antibodies
cloneBG-18, monoclonal
formbuffered aqueous glycerol solution
species reactivitybovine (non-reactive to IgM)
should not react with pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat
technique(s)direct ELISA: 1:25,000,dot blot: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG,western blot (chemiluminescent): 1:100,000
isotypeIgG1
shipped inwet ice
storage temp.2-8°C
target post-translational modificationunmodified
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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