MegaCell™ Dulbecco’s Modified Eagle’s Medium，500ML，M3942-500ML，Sigma

General description

MegaCell^™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Application

MegaCell^™ Dulbecco′s Modified Eagle′s Medium has been used:

• as a component of the M10 medium to culture CD133⁺ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
• as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
• to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Preparation Note

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Legal Information

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.