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General description
T-cell surface antigen CD2 (UniProt: P06729; also known as Erythrocyte receptor LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5) is encoded by the CD2 (also known as SRBC) gene (Gene ID: 914) in human. CD2 is a single-pass type I membrane glycoprotein that belongs to the immunoglobulin superfamily. It is composed of a single extracellular domain (aa 25-209) that contains two immunoglobulin-like domains (Ig-like domains), a transmembrane domain (aa 210-235) and a short cytoplasmic tail (aa 236-251). CD2 plays a vital role in T-cell activation thymocyte development, actin cytoskeleton rearrangement, and cellular signaling at the immunological synapse. It acts as a co-stimulatory molecule that enhances T-cell receptor (TCR) signaling upon binding to its ligands, such as CD58 on antigen-presenting cells. This interaction promotes T-cell proliferation, cytokine production, and overall immune response. CD2 is primarily expressed on T lymphocytes, including both CD4+ (helper) and CD8+ (cytotoxic) T cells. It is also found on natural killer (NK) cells, thymocytes and dendritic cells. In autoimmune diseases such as rheumatoid arthritis and multiple sclerosis, aberrant CD2 signaling may contribute to the dysregulation of T-cell responses. CD2 is expressed in certain hematological malignancies, including T-cell leukemias and lymphomas, where it may serve as a potential therapeutic target. Its role in enhancing T-cell activation also makes it a candidate for immunotherapy strategies aimed at boosting anti-tumor immunity. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Binder, C., et al., (2020), Front Immunol, 11, Article 1090)
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal half of human CD2.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human spleen tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD2 in human spleen tissue sections.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD2 in human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected CD2 in MOLT-4 cells.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged, recombinant fragment of human CD2.
Affinity Binding Assay: A representative lot of this antibody bound with CD2 peptide with at least one hundred thousand-fold (100,000X) higher than with non-specific control peptide.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD2 in one million human peripheral blood mononuclear cells (PBMCs).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1E21 is a ZooMAb® rabbit recombinant monoclonal antibody that detects CD2. It targets an epitope within 22 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1E21, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 40 kDa,observed mol wt ~35 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey, porcine |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_001315538 |
| UniProt accession no. | P06729 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... CD2(914) |
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