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General description
C-terminal-binding protein 2 (UniProt: P56545; also known as CtBP2) is encoded by the CTBP2 gene (Gene ID: 1488) in human. CTBP1 and CTBP2 are transcriptional co-repressors that modulate the activity of a large number of transcriptional repressors via the recruitment of chromatin modifiers. Although CTBP1 and CTBP2 show high degree of homology, their activity is differentially regulated, particularly through the control of their subcellular localization. It is only the CTBP2 that has a nuclear localization signal at its N-terminus. CTBP2 is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family that is ubiquitous in its distribution with highest levels observed in heart, skeletal muscle, and pancreas. It can exist as a homodimer or can form heterodimer with CTBP1. The repressor activity of CTBP2 is reported to be reciprocally regulated by NADH and acyl-CoA. It represses FoxO1-mediated gluconeogenesis directly and sterol regulatory element-binding protein 1 (SREBP1)-mediated lipogenesis indirectly. Obese subjects exhibit defective CTBP2 activity in their liver. Deletion of CTBP2 is shown to promote hepatic gluconeogenesis and enhance steatohepatitis. It is also involved in brown adipose tissue differentiation. A strict positive correlation between CTBP2 and T-cell lymphoma invasion and metastasis 1 (Tiam1) protein expression has been reported. Tiam1 serves as a downstream target of CTBP2. Knockdown of CTBP2 is reported to reduce Tiam1 levels resulting in lower invasion and migration. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sekiya, M., et al. (2021). Nat. Commun. 12; Article 6315; Paliwal, S., et al. (2012). Genes Cancer. 3(7-8); 481-490; Bergman, LM., et al. (2006). BMC Cell Biol. 7; Article 35).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human C-terminal-binding protein 2 (CTBP2).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CTBP2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CTBP2 in C6 and NIH3T3 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CTBP2 in A431, HeLa, HUVEC, and NIH3T3 cells.
Affinity Binding Assay: A representative lot of this antibody bound CTBP-2 peptide with a KD of 4.0 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CTBP2 in human gallbladder tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1C2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects C-terminal-binding protein 2 (CTBP2). It targets an epitope within 18 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1C2, recombinant monoclonal |
| description | 1C2 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 48.95 kDa,observed mol wt ~46 kDa |
| purified by | using Protein A |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | canine, monkey, bovine, porcine |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_001077383 |
| UniProt accession no. | P56545 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CTBP2(1488) |
| 长度(mm) | |
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