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Biochem/physiol Actions
H-Gly-Phe-AFC (GF-AFC) is a cell-permeable dipeptidyl peptidase 1 (DPP1; cathepsin C; cathepsin J) substrate commonly used for cell viability accessment, where the fluorophore AFC is cleaved off by intracellular DPP1 activity in live cells. Unlike ATP and MTT assays, GF-AFC assay requires no lysis at the end of the assay, so cells can be used afterwards for other live cell-based assays. Cells are typically incubated with GF-AFC at a final concentration of 80 µM (from a 100 mM stock in DMSO) for 0.5-1 h at 37°C, and the AFC fluorescence intensity can be monitored by a fluorescence plate reader or by a fluorescence microscope (Ex 380-400 nm and Em 505 nm).
Disclaimer
Hygroscopic, store with desiccant
| Quality Level | 100 |
| assay | ≥98% (HPLC) |
| form | powder |
| storage condition | desiccated |
| color | white to beige |
| solubility | H2O: 2 mg/mL, clear |
| storage temp. | -10 to -25°C |
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