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单克隆抗-磷酸酪氨酸，0.2ML，B1531-.2ML，Sigma

销售价： ¥ 5584.86 / 件

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订货号： 0BM1053

品牌型号： Sigma B1531-.2ML

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最小订货量： 1件

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商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

经 ELISA 和竞争性 ELISA 确定，该抗体与磷酸化的酪氨酸特异性反应，该磷酸化的酪氨酸既可以是游离氨基酸形式，也可与 BSA 或 KLH 等载体结合的形式。与非磷酸化的酪氨酸、磷酸苏氨酸、磷酸丝氨酸、AMP 或 ATP 没有观察到交叉反应。
Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine?biotin antibody can be used in dot blot (diluted 1:32,000). Mouse anti-phosphotyrosine?biotin antibody reacts specifically with phosphorylated tyrosine both as the free amino acid or when conjugated to BSA or KLH. This product does not react with non-phosphorylated tyrosine or other phosphorylated amino acids, including serine and threonine, or with phosphorylated molecules like AMP or ATP.

Immunogen

与 BSA 结合的磷酸酪氨酸

Application

Monoclonal Anti-Phosphotyrosine?Biotin antibody produced in mouse has been used in western blot analysis to detect tyrosine phosphorylated proteins. It has also been used in BIAcore analysis. This conjugate maybe used as an analytical tool by enabling the identification and quantification of tyrosine phosphorylated proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	mouse
Quality Level	200
conjugate	biotin conjugate
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	PT-66, monoclonal
form	buffered aqueous solution
technique(s)	direct ELISA: 1:50,000,dot blot: 1:32,000
isotype	IgG1
shipped in	dry ice
storage temp.	−20°C
target post-translational modification	unmodified

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