- 商品介绍
- 规格参数
- 包装参数
General description
Integrin aV (UniProt: P06756; also known as Vitronectin receptor, Vitronectin receptor subunit alpha, CD51) is encoded by the ITGAV (also known as MSK8, VNRA, VNTR) gene (Gene ID: 3685) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and a b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the an a subunit. The structural and functional diversity of the integrin family is based upon the pairing abilities of the individual a and b subunits. Integrins are not constitutively active and their activation from a low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrin aV is a single-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 31-992), a transmembrane domain (aa 993-1016), and a cytoplasmic domain (aa 1017-1048). The a-subunit is composed of a heavy (aa 31-889) and a light (aa 891-1048) chain linked by a disulfide bond. Integrin aV can associate with b-1, b b-3, b-5, b-6, or b-8 in a non-covalent manner. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Immunogen
Full-length purified human Integrin aV.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in A549 cell line.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin aV in A549 cells.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin V in human kidney and human placenta tissue sections.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Integrin V in one million A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Integrin aV, clone 272-17E6 ZooMAb®, Cat. No. ZMS1053, is a recombinant Mouse monoclonal antibody that specifically targets Integrin aV and is tested for use in Flow Cytometry, Immunocytochemistry, and Immunohistochemistry (Paraffin).
Biochem/physiol Actions
Clone 272-17E6 is a a ZooMAb® mouse recombinant monoclonal antibody that specifically detects human Integrin aV.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 272-17E6, recombinant monoclonal,monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt ~116.04 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| isotype | IgG1κ |
| UniProt accession no. | P06756 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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