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General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Protein Wnt-3a (UniProt: P56704) is encoded by the WNT3A gene (Gene ID: 89780) in human. The evolutionarily conserved Wnt signaling pathway is involved in cell proliferation, fate and polarity determination during embryonic development, and in cell migration. Abnormalities in Wnt signaling are reported to promote birth defects, degenerative diseases, and cancer. Wnt genes encode a large family of secreted, cysteine-rich proteins of approximately 350 to 400 amino acids (~40 kDa) that are important in development and maintenance of adult tissues. Nineteen different Wnt proteins have been identified in mammals thus far. They are either tethered to the plasma membrane or exit the cell via multiple routes, including direct release from the plasma membrane by solubilization and the formation of exosomes. Wnt-3a serves as a ligand for the frizzled family of transmembrane receptors. It induces the accumulation of β-catenin, thereby activating the canonical Wnt signaling pathway that results in the activation of transcription factors of the TCF/LEF family. Wnt-3a can promote or suppress tumor progression via the canonical Wnt signaling pathway depending on cancer type. Wnt-3a treatment is reported to provide neuroprotection and promote neurogenesis and improve neurocognitive function in traumatic brain injury murine models. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mihara, E., et al. (2016). Elife. 5; e1162; Zhao, Y., et al. (2016). Stem Cells. 34(5); 1263-1272; Pollheimer, J., et al. (2006). Am. J.Pathol. 168(4); 1134-1147).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human WNT3A.
Application
Quality Control Testing
Evaluated by Western Blotting in WNT3A conditioned media.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Wnt3a in WNT3A conditioned media.
Tested Applications
Western Blotting Analysis: A 1:1.000 dilution of this antibody detected recombinant fragment of human WNT3A.
Enzyme Immunoassay: A representative lot of this antibody detected WNT3A protein in ELISA application.
Affinity Binding Assay: A representative lot of this antibody bound WNT3A peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2I8 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects WNT3A. It targets an epitope within 18 amino acids from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2I8, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 39.37 kDa,observed mol wt ~40 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse, rat |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_149122.1 |
| UniProt accession no. | P56704 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... WNT3A(89780) |
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