Alpaca Anti-Rabbit IgG(Fcγ) Biotin single domain Antibody,100UG,SAB5801056-100UG,Sigma

销售价: ¥ 4393.04 / 件
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订货号 0BM6393
品牌型号 Sigma SAB5801056-100UG
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Alpaca Anti-Rabbit IgG(Fcγ) Biotin single domain Antibody is designed to specifically detect Fc region of rabbit IgG. It is a monovalent, single domain, recombinant antibody to rabbit IgG (Fcγ Fragment specific) coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Rabbit IgG(Fcγ) Biotin single domain Antibody reacts with the Fc region of rabbit IgG selectively but not with mouse, human, cynomolgus, rat, goat IgG.

Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.


Immunogen

Recombinant Alpaca single variable domain secondary nano antibody


Biochem/physiol Actions

Fc region of Rabbit IgG
Isotype: VHH domain of alpaca IgG2b/2c


Packaging

100 μg in Glass bottle


Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose


Preparation Note

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C


Other Notes

No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG


技术参数 Specifications
biological sourcealpaca
recombinantexpressed in E. coli
conjugatebiotin
antibody formaffinity purified immunoglobulin
antibody product typesecondary antibodies
clonerecombinant monoclonal
formlyophilized solid
mol wt 14 kDa
species reactivityrabbit, Alpaca
technique(s)ELISA: 1:10000-1:50000,western blot: 1:10000-1:50000
size0.1 mL
color colorless
isotypeVHH
shipped inwet ice
storage temp.2-8°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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