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General description
GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10.
Immunogen
synthetic peptide corresponding to the C-terminal region of human GAD 65 (amino acids 514-530). The sequence is identical in mouse GAD 65 and highly conserved in rat and pig GAD 65 but not found in GAD 67.
Application
Anti-Glutamic Acid Decarboxylase 65 antibody produced in rabbit is suitable as a primary antibody for the determination of expression of GAD 65 isoform in putative P boutons in rat and mouse at a working dilution of 1:5000 and in lysates prepared from rat testis at different developmental ages and rat cerebellum by immunoblotting.
It is also suitable for immunohistochemistry at a working dilution of 1:500 using formalin-fixed, paraffin-embedded sections of rat pancreas and for microarray.
Biochem/physiol Actions
Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release.
Reacts specifically with GAD 65 (65 kDa) from brain.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| species reactivity | mouse, pig, human, rat |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using tissue sections of rat pancreas,microarray: suitable,western blot: 1:4,000 using rat brain extract |
| UniProt accession no. | Q05329 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... GAD2(2572) mouse ... Gad2(14417) rat ... Gad2(24380) |
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