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General description
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues.
Biochem/physiol Actions
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in E. coli |
| assay | ≥98% (SDS-PAGE) |
| form | lyophilized |
| mol wt | 15.1 kDa |
| packaging | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white |
| UniProt accession no. | ,P01586 |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information | mouse ... IL3(16187) |
| 长度(mm) | |
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