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General description
Chromodomain-helicase-DNA-binding protein 8 (UniProt: Q9HCK8; also known as EC: 3.6.4.12, CHD-8, ATP-dependent helicase CHD8, Helicase with SNF2 domain 1) is encoded by the CHD8 (also known as HELSNF1, KIAA1564) gene (Gene ID: 57680) in human. CHD-8 is a member of the SNF2/RAD54 helicase family. Two isoforms of CHD-8 have been reported that are produced by alternative splicing. It is a DNA helicase that acts as a chromatin remodeling factor and regulates transcription. It also acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. CHD-8 is reported to suppress p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity and also acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin activity. It negatively regulates β-catenin-targeted gene expression by being recruited specifically to the promoter regions of several β-catenin responsive genes. CHD-8 is involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Mutations in CHD8 gene are associated with Autism 18 that is characterized by impairments in reciprocal social interaction and communication and developmental abnormalities. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Xu, Q., et al. (2018). Mol. Autism. 9; Article 65; Thompson, BA., et al. (2008). Mol. Cell Biol. 28(12); 3894-3904).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 20 amino acids from the internal region of human Chromodomain-helicase-DNA-binding protein 8 (CHD-8).
Application
Quality Control Testing
Evaluated by Western Blotting in EL4 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHD-8 in EL4 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CHD-8 in nuclear extracts from NIH3T3 and HeLa cells.
Affinity Binding Assay: A representative lot of this antibody bound CHD-8 peptide with a KD of 5.1 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CHD-8 in human prostate tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in EL4 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHD-8 in EL4 cell lysate.
Biochem/physiol Actions
Clone 1H9 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Chromodomain-helicase-DNA-binding protein 8 (CHD-8). It targets an epitope within 20 amino acids from the internal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1H9, recombinant monoclonal |
| description | 1H9 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 290.52 kDa,observed mol wt ~290 kDa |
| purified by | using Protein A |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_001164100 |
| UniProt accession no. | Q9HCK8 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CHD8(57680) |
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