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General description
Beta-galactosidase (UniProt: P00722; also known as EC:3.2.1.23; Beta-gal) is encoded by the LacZ gene (Gene ID: 945006) in E. coli. The β-galactosidase is a tetrameric enzyme composed of four polypeptide chains (A-D), each with 1023 amino acids. Each monomer contains five domains (1-5). Domain 3 has an /β or TIM barrel structure with the active site located at the C-terminal end of the barrel. The residues that form the active site are contributed by different segments of the polypeptide chain. β-galactosidase hydrolyzes the disaccharide lactose to generate galactose and glucose and can also convert lactose to allolactose, which is the natural inducer for the lac operon. It displays strict specificity at the galactosyl position but can hydrolyze β-D-galactopyranosides with a wide variety of aglycons. Its substrate binding site is localized to amino acids 538-571. The hydrolysis reaction is a double displacement reaction where the product retains the same stereochemistry as the starting state. In the initial step, the substrate forms a covalent -D-galactosyl enzyme intermediate with the nucleophile Glu537. In the second step the intermediate is released, a step that is facilitated by Glu461, which abstracts a proton from the acceptor molecule, R OH. The activity of β-galactosidase is dependent upon the presence of divalent (Mg2+) and monovalent (Na+) cations. Mg2+ causes a 5-to-100-fold activation, depending on the substrate. However, Mg2+ can be replaced by Mn2+ to obtain full activity. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mathews, BW. (2005). CR Biologics. 328(6); 549-556; Juers, DH., et al. (2001). Biochemistry. 40(49); 14781-14794).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of E.coli β-galactosidase.
Application
Quality Control Testing
Evaluated by Western Blotting with E. coli β-Galactosidase.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected E.coli β-Galactosidase.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected β-Galactosidase in HeLa cells infected with Adenovirus expressing β-Galactosidase.
Enzyme Immunoassay Analysis (ELISA): A serial of dilutions from a representative lot detected E. coli β-Galactosidase.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with E. coli β-Galactosidase.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected E.coli β-Galactosidase.
Biochem/physiol Actions
Clone 1G21 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects E. coli β-Galactosidase. It targets an epitope within 24 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1G21, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 116.48 kDa,observed mol wt ~120 kDa |
| purified by | using Protein A |
| species reactivity | E. coli |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_414878 |
| UniProt accession no. | P00722 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | bacterial ... LacZ(945006) |
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