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General description
Goat IgG is a glycoprotein antibody that is fractionated from pooled normal goat serum. In immunoelectrophoresis, goat IgG yields a single major arc of precipitation in the γ region against anti-goat IgG and anti-goat whole serum. Minor γ arcs may also be observed against anti-goat whole serum.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Goat IgG is purified from normal goat serum by fractionation.
Application
In immunohistochemical applications, IgG from goat serum can be used as a blocking agent (500 μg/ml in paraformaldehyde-fixed mouse uterus sections) or as a negative control (1:56000 in pig cecal tissue).
Technical grade IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. Goat IgG was used as negative control antibody in immunohistochemical staining of cecal tissue of pigs at a dilution of 1:56000. Goat IgG was used in immunohistochemistry of rat brain sections at a concentration of 2 μg/ml. The antibody was also used in chromatin immunoprecipitation and as control in infection inhibition assay.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.2 containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| conjugate | unconjugated |
| Quality Level | 200 |
| grade | technical grade |
| assay | ≥80% (SDS-PAGE) |
| form | buffered aqueous solution |
| technique(s) | ChIP: suitable,immunohistochemistry: suitable |
| shipped in | dry ice |
| storage temp. | −20°C |
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