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General description
The 2,2,7-trimethylguanosine (TMG, m3G) cap structure is an important functional characteristic of ncRNAs with critical cellular roles. It is one of the earliest identified RNA modifications that ensures nuclear localization of small nuclear RNAs (snRNAs). It affects pre-mRNA synthesis/splicing, RNA cytoplasmic transport, and mRNA translation and turnover. Shortly after transcription, 7-methylguanosine (m7G), known as cap structure, is added to 5 -end of newly synthesized U snRNA and m7G-capped U snRNA is exported to the cytoplasm. The m7G-cap is converted to m3G-cap, which is a hypermethylated structure of 5 -end, by cytoplasmic catalytic protein TSG1 and the m3G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. Capping of RNAs is an important requirement for RNA transport and splicing processes. Uncapped RNAs are rapidly degraded by the 5 exoribonuclease enzyme. The TMG-cap also plays a key role in recruiting telomerase to telomeres and engaging Cajal bodies in telomere maintenance. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yedavalli, YSRK., and Jeang, K-T. (2010). Proc. Natl. Acad. Sci. USA. 107(33); 14787-14792; Moreno, PMD., et al. (2009). Nucleic Acids Res. 37(6); 1925-1935).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated 2,2,7-trimethylguanosine
Application
Quality Control Testing
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected m3G/TMG cap in HeLa cells.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected m3G/TMG cap in A431, HepG2, and NIH3T3 cells.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected m3G/TMG cap in Human spleen tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected m3G/TMG cap in Human spleen and Human cervix tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone K121 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects m3G/TMG.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | K121, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| purified by | using protein G |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | all |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable |
| isotype | IgG1κ |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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