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General description
EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4neu. EGFR can heterodimerize with each of the members of this family.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Immunogen
Recombinant human EGFR (Leu25-Ser645) protein
Application
ELISA, IHC,IF
Biochem/physiol Actions
Human Epidermal Growth Factor Receptor 1 (HER1/EGFR)
Packaging
50 µg per vial / 100 µg per vial
Physical form
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.9
Preparation Note
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Stable for 1 year at 4°C from date of receipt.
Other Notes
Storage at 4°C; Do not freeze.
| biological source | alpaca |
| Quality Level | 100 |
| recombinant | expressed in E. coli |
| conjugate | unconjugated |
| antibody form | affinity purified VHH antibody |
| clone | EB61, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | lyophilized solid |
| mol wt | 14.4 kDa, ~15-18 kDa (SDS PAGE under reducing condition) |
| purified by | immobilized metal affinity chromatography (IMAC) |
| species reactivity | human |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:1000-1:25000,immunofluorescence: 1:200-1:600,immunohistochemistry: 1:200-1:600 |
| color | colorless |
| pH | ( in PBS, pH 7.4) |
| isotype | VHH |
| Protein ID accession no. | AAI18666.1 |
| UniProt accession no. | P00533 |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... EGFR(1956) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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